atf3 protein levels Search Results


odyssey imaging system  (LI-COR)
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LI-COR odyssey imaging system
Odyssey Imaging System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atf3 protein levels  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology atf3 protein levels
Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with <t>pCMV6-ATF3,</t> a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
Atf3 Protein Levels, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tripeptide glycine–proline–glutamate analogue nnz-2566  (Neuren Inc)
90
Neuren Inc tripeptide glycine–proline–glutamate analogue nnz-2566
Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with <t>pCMV6-ATF3,</t> a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
Tripeptide Glycine–Proline–Glutamate Analogue Nnz 2566, supplied by Neuren Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human atf3 enzyme  (Aviva Systems)
93
Aviva Systems human atf3 enzyme
Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with <t>pCMV6-ATF3,</t> a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
Human Atf3 Enzyme, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kits  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology elisa kits
Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with <t>pCMV6-ATF3,</t> a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
Elisa Kits, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dual-luciferase reporter assay system kit  (Promega)
90
Promega dual-luciferase reporter assay system kit
Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with <t>pCMV6-ATF3,</t> a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
Dual Luciferase Reporter Assay System Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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camp/proteinkinase a (pka)/camp-response element binding protein (creb) axis  (ProteinKinase)
90
ProteinKinase camp/proteinkinase a (pka)/camp-response element binding protein (creb) axis
Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with <t>pCMV6-ATF3,</t> a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
Camp/Proteinkinase A (Pka)/Camp Response Element Binding Protein (Creb) Axis, supplied by ProteinKinase, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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deoxynucleotide-triphosphates  (Promega)
90
Promega deoxynucleotide-triphosphates
Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with <t>pCMV6-ATF3,</t> a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
Deoxynucleotide Triphosphates, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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random primers  (Promega)
90
Promega random primers
Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with <t>pCMV6-ATF3,</t> a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
Random Primers, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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odyssey  (LI-COR)
99
LI-COR odyssey
Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with <t>pCMV6-ATF3,</t> a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
Odyssey, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p38  (Affinity Biosciences)
86
Affinity Biosciences p38
Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with <t>pCMV6-ATF3,</t> a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
P38, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goatarabbit irdye 800cw  (LI-COR)
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LI-COR goatarabbit irdye 800cw
Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with <t>pCMV6-ATF3,</t> a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments
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Image Search Results


Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with pCMV6-ATF3, a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments

Journal: Virology journal

Article Title: Activating transcription factor 3 induces oxidative stress and genotoxicity, transcriptionally modulating metastasis-related gene expression in human papillomavirus-infected cervical cancer.

doi: 10.1186/s12985-025-02675-0

Figure Lengend Snippet: Fig. 1 Analysis of fluorescence intensity in HeLa and Ca Ski cells transfected with pCMV6-ATF3, a mock vector, or left untreated as controls, utilizing fluorescence microscopy and flow cytometry at 48 and 72 h post-transfection. (A) Fluorescent imaging of HeLa and Ca Ski cells was performed at 20x magnification, comparing cells transfected with pCMV6-ATF3 to mock and control groups. (B) Mean fluorescence intensity levels were measured across pCMV6-ATF3, mock, and control samples. Flow cytometry results showed transfection efficiencies of 89.41% in HeLa and 88.13% in Ca Ski cells at 72 h post-transfection, significantly higher than the mock and control groups (p < 0.0001). (C) Western blot analysis was conducted to assess ATF3 protein ex pression in HeLa and Ca Ski cell lysates, using anti-ATF3 and anti-β-actin antibodies. (D) Densitometry of ATF3 expression levels showed a marked increase in cells transfected with pCMV6-ATF3 compared to control and mock groups (p < 0.001). Results are presented as the mean ± SD from three independent experiments

Article Snippet: ATF3 protein levels were detected using a monoclonal anti-ATF3 antibody (1:500, Santa Cruz Biotechnologies). β-actin (1:5000, Santa Cruz Biotechnologies) was used as a loading control.

Techniques: Fluorescence, Transfection, Plasmid Preparation, Microscopy, Flow Cytometry, Imaging, Control, Western Blot, Expressing

Fig. 2 Effect of ATF3 overexpression on the expression of HPV-16 and HPV-18 E6/E7, MMP1, and SHARP1 genes in HeLa (A) and Ca Ski (B) cells, analyzed by RT-qPCR. (A) In HeLa cells, overexpression of ATF3 resulted in a significant decrease in MMP1 gene expression (p < 0.01), while SHARP1 and HPV-18 E6/ E7 mRNA levels remained largely unaffected (p > 0.05) compared to mock and control groups. (B) Similarly, in Ca Ski cells, ATF3 overexpression significantly reduced MMP1 expression (p < 0.02), but SHARP1 and HPV-18 E6/E7 expression levels showed no significant difference (p > 0.05) relative to mock and control groups. Both HeLa and Ca Ski cells transfected with pCMV6-ATF3 demonstrated markedly higher ATF3 mRNA expression compared to mock and control groups (p < 0.001). Data are presented as the mean ± SD from three independent experiments

Journal: Virology journal

Article Title: Activating transcription factor 3 induces oxidative stress and genotoxicity, transcriptionally modulating metastasis-related gene expression in human papillomavirus-infected cervical cancer.

doi: 10.1186/s12985-025-02675-0

Figure Lengend Snippet: Fig. 2 Effect of ATF3 overexpression on the expression of HPV-16 and HPV-18 E6/E7, MMP1, and SHARP1 genes in HeLa (A) and Ca Ski (B) cells, analyzed by RT-qPCR. (A) In HeLa cells, overexpression of ATF3 resulted in a significant decrease in MMP1 gene expression (p < 0.01), while SHARP1 and HPV-18 E6/ E7 mRNA levels remained largely unaffected (p > 0.05) compared to mock and control groups. (B) Similarly, in Ca Ski cells, ATF3 overexpression significantly reduced MMP1 expression (p < 0.02), but SHARP1 and HPV-18 E6/E7 expression levels showed no significant difference (p > 0.05) relative to mock and control groups. Both HeLa and Ca Ski cells transfected with pCMV6-ATF3 demonstrated markedly higher ATF3 mRNA expression compared to mock and control groups (p < 0.001). Data are presented as the mean ± SD from three independent experiments

Article Snippet: ATF3 protein levels were detected using a monoclonal anti-ATF3 antibody (1:500, Santa Cruz Biotechnologies). β-actin (1:5000, Santa Cruz Biotechnologies) was used as a loading control.

Techniques: Over Expression, Expressing, Quantitative RT-PCR, Gene Expression, Control, Transfection

Fig. 3 Evaluation of ROS levels in HeLa and Ca Ski cells following pCMV6-ATF3 transfection. (A) Flow cytometry histograms illustrating ROS levels in HeLa and Ca Ski cells, measured with the DCHF-DA assay. (B) Quantitative comparison shows a significant rise in ROS levels in pCMV6-ATF3-transfected cells relative to mock and control groups (p < 0.01). Results are shown as the mean ± SD from three separate experiments

Journal: Virology journal

Article Title: Activating transcription factor 3 induces oxidative stress and genotoxicity, transcriptionally modulating metastasis-related gene expression in human papillomavirus-infected cervical cancer.

doi: 10.1186/s12985-025-02675-0

Figure Lengend Snippet: Fig. 3 Evaluation of ROS levels in HeLa and Ca Ski cells following pCMV6-ATF3 transfection. (A) Flow cytometry histograms illustrating ROS levels in HeLa and Ca Ski cells, measured with the DCHF-DA assay. (B) Quantitative comparison shows a significant rise in ROS levels in pCMV6-ATF3-transfected cells relative to mock and control groups (p < 0.01). Results are shown as the mean ± SD from three separate experiments

Article Snippet: ATF3 protein levels were detected using a monoclonal anti-ATF3 antibody (1:500, Santa Cruz Biotechnologies). β-actin (1:5000, Santa Cruz Biotechnologies) was used as a loading control.

Techniques: Transfection, Flow Cytometry, Comparison, Control

Fig. 4 DNA damage analysis by comet assay using alkaline single-cell gel electrophoresis. (A) Representative images from the comet assay display DNA migration patterns in HeLa and Ca Ski cells post-transfection with pCMV6-ATF3. In these images, intact DNA forms a distinct head, while fragmented DNA trails behind, forming a comet tail—longer tails signify greater DNA damage. (B) Quantification of comet assay results for HeLa and Ca Ski cells across pCMV6-ATF3-transfected, mock-transfected, and control groups. Minimal DNA damage was observed in both control and mock-transfected cells. Data are expressed as the mean ± SD from three independent replicates

Journal: Virology journal

Article Title: Activating transcription factor 3 induces oxidative stress and genotoxicity, transcriptionally modulating metastasis-related gene expression in human papillomavirus-infected cervical cancer.

doi: 10.1186/s12985-025-02675-0

Figure Lengend Snippet: Fig. 4 DNA damage analysis by comet assay using alkaline single-cell gel electrophoresis. (A) Representative images from the comet assay display DNA migration patterns in HeLa and Ca Ski cells post-transfection with pCMV6-ATF3. In these images, intact DNA forms a distinct head, while fragmented DNA trails behind, forming a comet tail—longer tails signify greater DNA damage. (B) Quantification of comet assay results for HeLa and Ca Ski cells across pCMV6-ATF3-transfected, mock-transfected, and control groups. Minimal DNA damage was observed in both control and mock-transfected cells. Data are expressed as the mean ± SD from three independent replicates

Article Snippet: ATF3 protein levels were detected using a monoclonal anti-ATF3 antibody (1:500, Santa Cruz Biotechnologies). β-actin (1:5000, Santa Cruz Biotechnologies) was used as a loading control.

Techniques: Single Cell Gel Electrophoresis, Alkaline Single Cell Gel Electrophoresis, Migration, Transfection, Control